Concordance of the frequency, typing, and results of high risk human papilloma virus in cervical cytology materials with biopsy: Retrospective analysis of 5604 patients
Ebru Akay, Fatoş Tekelioglu, Hatice Karaman
Kayseri City Hospital, Clinic of, Pathology, Kayseri, Turkey
Aim: The aim of the study is to evaluate retrospectively the results of human papilloma virüs (HPV) screening test started at our center, to identify the rates of high risk HPV (hrHPV) causing /not causing cellular anomalies and HPV16, 18/45 genotypes, to review the concordance of smear results and biopsy, and to compare our results with the similar studies conducted in different parts of the world and our country.
Material and Methods: Five thousand six hundred four cases in Hospital, between 2016 and 2017, with transcription-mediated amplification and hybridization protection method,14 hrHPV types including HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 were investigated with presence of HPV E6/E7 mRNA. HPV(+) cases were reevaluated with the genotyping study for detection of HPV16, 18/45.
Results: When the hrHPV results of 5604 patients were evaluated, 421 cases (7.5%) were hrHPV positive and 5183 cases (92.5%) were hrHPV negative. In the genotyping study of hrHPV positive cases, it was found that HPV16 was present in 97 cases (23%), HPV18/45 was present in 40 cases (9.5%) and other hrHPVs were found in 282 cases (67%). HPV16 and HPV 18/45 coexisted in 2 cases (0.5%).
Biopsy results of hrHPV positive cases were 56 cases (32.7%), 40 cases (23.4%) and 5 cases (2.9%) for low grade squamous intraepithelial lesion (LGSIL), high grade squamous intraepithelial lesion (HGSIL) and Squamous Cell Carcinoma (SCC), respectively. Conclusion: In our study, prevalence of hrHPV is 1.2% in cases with normal cytology results and 7.5% in general population. In the majority of studies conducted in our country, HPV positivity is very high because HPV is studied in smear and cervical anomaly cases instead of general population.
We believe that this study contributes to determine the frequency and genotype distribution in normal/ abnormal cytology of HPV in our country.
Keywords: HPV; Cervix; Pap Smear; Cervical Intraepithelial Neoplasia.